| 課程編碼 Course Code | 中文課程名稱 Course Name (Chinese) | 英文課程名稱 Course Name (English) | 總學分數 Credits | 總時數 Hours |
|---|---|---|---|---|
| 3503013 | 生物化學實驗 | Biochemistry experiments | 2.0 | 4 |
| 中文概述 Chinese Description | 本實驗課程是學習設計引子與利用聚合?連鎖反應來放大並取DNA片段，並以重組技術來選殖DNA。瞭解限制?可在雙股DNA特定序列做切割、連接?可將DNA的5’與3’兩端連接。學習讓DNA轉殖進入勝任細胞、培養細胞並純化蛋白質、測定其功能及培養晶體。 | |||
| 英文概述 English Description | The aim of this course is to introduce a series of biotechniques. To amplify a certain DNA fragment by using PCR and to clone DNA by using Recombinant DNA technology�r. Different restriction enzymes can cleave at double strand DNA at specific sites, T4 DNA ligase does link 5’and 3’ of DNA. The amplified DNA were inserted onto plasmid and then transformed into host cells and culture the cell. Learning how to express protein and analyze function as well as to grow crystal. | |||
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