Course Syllabus

Course Information：

Year-Semester Course Number Course Name Phase Credits Hours Required/Elective Instructor Class Student NO. Withdraw Note

Spring Semester
Year 2024 331872 Protein Science and Glyciology 1 3.0 3 ★ KUO-YUAN HWA GOPM 0 0 英文上課

Year-Semester	Course Number	Course Name	Phase	Credits	Hours	Required/Elective	Instructor	Class	Student NO.	Withdraw	Note
Spring Semester Year 2024	331872	Protein Science and Glyciology	1	3.0	3	★	KUO-YUAN HWA	GOPM	0	0	英文上課

Course Plan：

Instructor KUO-YUAN HWA　Office Hours

Email kyhwa@ntut.edu.tw

Last Updated 2024-02-19 12:58:30

Course Objective The course is designed to teach upper level graduate students about the recent advances in protein science and glycobiology with specific application in medical and biotechnological industries. The prerequisite classes for this course are: general organic chemistry, biochemistry, protein structure and glycobiology. Students are expected to learn the structure, biosynthesis, chemistry and cell biology in related to bioactive protein and glycconjugates. Students must be highly motivated and required actively participated in class discussion.

Course Schedule Week/ Date Title 1. Central dogma 2. Amino acid, peptides & proteins 3. Protein Structure and Function 4. Biochemical Pathway and enzyme 5. Protein purification 6. Protein purification 7. Gel & Western Analysis 8 LC & Mass-spec analysis 9. Mid-term case report (30%) 10. Introduction on glycobiology 11. Types & structures of glycan 12 Biosynthesis-N-glycans 13. Biosynthesis-O-linked glycans & O-GlcNAc 14. Biosynthesis of GPI 15. Proteoglycan & GAG 16. Structural analysis of glycans 17. Structural analysis of glycans 18. Final case report ( 50%)

Evaluation and grading policy Evaluation: Homework (20%), Mid-term (30 %), Final case report (40%), Class attendance (10 %) There will be 4 homework assignments. Mid-term presentation: PPT file Final exam is a summary report on selective topics.

Materials All right reserved. Do not reproduce without permission.

Foreign-language textbooks:Yes
Proteins: Concepts in Biochemistry, Garland Science, 1st edition (2016) by Paulo Almei https://www.amazon.com/-/zh_TW/Paulo-Almeida-ebook/dp/B01D0KEV4W/ref=sr_1_1?crid=3A09KY7MOFOCY&keywords=Proteins%3A+Concepts+in+Biochemistry%2C+Garland+Science&qid=1641201621&s=digital-text&sprefix=proteins+concepts+in+biochemistry%2C+garland+science%2Cdigital-text%2C241&sr=1-1 Open Textbook: Biochemistry: Free For All https://open.umn.edu/opentextbooks/textbooks/biochemistry-free-for-all-ahern The Sugar Code: Fundamentals of Glycosciences, 1st edition ( ) by Hans-Joachim Gabius https://www.amazon.com/-/zh_TW/Hans-Joachim-Gabius-ebook-dp-B0063CWBTM/dp/B0063CWBTM/ref=mt_other?_encoding=UTF8&me=&qid=

The access to curricular consultation Students are welcome during my office hour, Tue or Wed 9:30 a.m. to 11:30 a.m.
Or leave a message in the Team group.

The course corresponds to the SDGs

Note
We will use Microsoft Team for the class.

Register and download the software:
https://www.microsoft.com/zh-tw/microsoft-teams/group-chat-software

Instructor	KUO-YUAN HWA　Office Hours
Email	kyhwa@ntut.edu.tw
Last Updated	2024-02-19 12:58:30
Course Objective	The course is designed to teach upper level graduate students about the recent advances in protein science and glycobiology with specific application in medical and biotechnological industries. The prerequisite classes for this course are: general organic chemistry, biochemistry, protein structure and glycobiology. Students are expected to learn the structure, biosynthesis, chemistry and cell biology in related to bioactive protein and glycconjugates. Students must be highly motivated and required actively participated in class discussion.
Course Schedule	Week/ Date Title 1. Central dogma 2. Amino acid, peptides & proteins 3. Protein Structure and Function 4. Biochemical Pathway and enzyme 5. Protein purification 6. Protein purification 7. Gel & Western Analysis 8 LC & Mass-spec analysis 9. Mid-term case report (30%) 10. Introduction on glycobiology 11. Types & structures of glycan 12 Biosynthesis-N-glycans 13. Biosynthesis-O-linked glycans & O-GlcNAc 14. Biosynthesis of GPI 15. Proteoglycan & GAG 16. Structural analysis of glycans 17. Structural analysis of glycans 18. Final case report ( 50%)
Evaluation and grading policy	Evaluation: Homework (20%), Mid-term (30 %), Final case report (40%), Class attendance (10 %) There will be 4 homework assignments. Mid-term presentation: PPT file Final exam is a summary report on selective topics.
Materials	All right reserved. Do not reproduce without permission. Foreign-language textbooks:Yes Proteins: Concepts in Biochemistry, Garland Science, 1st edition (2016) by Paulo Almei https://www.amazon.com/-/zh_TW/Paulo-Almeida-ebook/dp/B01D0KEV4W/ref=sr_1_1?crid=3A09KY7MOFOCY&keywords=Proteins%3A+Concepts+in+Biochemistry%2C+Garland+Science&qid=1641201621&s=digital-text&sprefix=proteins+concepts+in+biochemistry%2C+garland+science%2Cdigital-text%2C241&sr=1-1 Open Textbook: Biochemistry: Free For All https://open.umn.edu/opentextbooks/textbooks/biochemistry-free-for-all-ahern The Sugar Code: Fundamentals of Glycosciences, 1st edition ( ) by Hans-Joachim Gabius https://www.amazon.com/-/zh_TW/Hans-Joachim-Gabius-ebook-dp-B0063CWBTM/dp/B0063CWBTM/ref=mt_other?_encoding=UTF8&me=&qid=
The access to curricular consultation	Students are welcome during my office hour, Tue or Wed 9:30 a.m. to 11:30 a.m. Or leave a message in the Team group.
The course corresponds to the SDGs
Note	We will use Microsoft Team for the class. Register and download the software: https://www.microsoft.com/zh-tw/microsoft-teams/group-chat-software