Course Description

Course CodeCourse NameCreditsHours
3503013 Biochemistry experiments 2.0 4
Description The aim of this course is to introduce a series of biotechniques. To amplify a certain DNA fragment by using PCR and to clone DNA by using Recombinant DNA technology. Different restriction enzymes can cleave at double strand DNA at specific sites, T4 DNA ligase does link 5’and 3’ of DNA. The amplified DNA were inserted onto plasmid and then transformed into host cells and culture the cell. Learning how to express protein and analyze function as well as to grow crystal.